LeHavreV1, France, Analysis, bibRecord, 000E94

Determination of Glycols in Biological Specimens by Gas Chromatography-Mass Spectrometry

Identifieur interne : 000E94 ( France/Analysis ); précédent : 000E93; suivant : 000E95

Determination of Glycols in Biological Specimens by Gas Chromatography-Mass Spectrometry

Auteurs : Vincent Gembus [France] ; Jean-Pierre Goullé [France] ; Christian Lacroix [France]

Source :

Journal of Analytical Toxicology [ 0146-4760 ] ; 2002-07.

RBID : ISTEX:9BAA3DF14449C42AF714C39FF83E26EEEDFCDE73

Abstract

A simple extraction and derivatization procedure for the analysis of eight glycols (ethylene glycol, EG; diethylene glycol, DEG; triethylene glycol, TEG; 1,2-propanediol, 1,2-PD; 1,3-propanediol, 1,3-PD; 1,2-butanediol, 1,2-BD; 2,3-butanediol, 2,3-BD; and hexylene glycol, HXG) using a 2-µL serum or blood sample is described. Following deproteinisation with acetonitrile, derivatization to its mono or di TMS derivative, glycols were detected using gas chromatography-electron impact mass spectrometry equipped with a split-spitless inlet and a DB-5MS column in the scan mode from 40 to 500 amu. Gammahydroxybutyrate-d6 (GHB-d6) was used as the internal standard. The limits of detection and quantitation in 2 µL of serum ranged, respectively, from 0.7 mg/L for EG to 8.5 mg/L for TEG and from 1.3 mg/L for EG to 18.2 mg/L for 1,2-PD. A linear response was observed over the concentration range from 1 to 800 mg/L for EG and 18 from 800 for TEG and 1,2-PD for serum and blood. Coefficients of variation for both intra-assay precision and interassay reproductibility ranged respectively between 1.9% for TEG to 4.9% for 1,2-PD (11.8% for HXG) and 3.5% for DEG to 9% for 2,3-BD (20.4 for HXG) at the 400 mg/L serum level. The method was applied to plasma and whole blood.

Url:

https://api.istex.fr/document/9BAA3DF14449C42AF714C39FF83E26EEEDFCDE73/fulltext/pdf

DOI: 10.1093/jat/26.5.280

Affiliations:

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ISTEX:9BAA3DF14449C42AF714C39FF83E26EEEDFCDE73

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<front><div type="abstract">A simple extraction and derivatization procedure for the analysis of eight glycols (ethylene glycol, EG; diethylene glycol, DEG; triethylene glycol, TEG; 1,2-propanediol, 1,2-PD; 1,3-propanediol, 1,3-PD; 1,2-butanediol, 1,2-BD; 2,3-butanediol, 2,3-BD; and hexylene glycol, HXG) using a 2-µL serum or blood sample is described. Following deproteinisation with acetonitrile, derivatization to its mono or di TMS derivative, glycols were detected using gas chromatography-electron impact mass spectrometry equipped with a split-spitless inlet and a DB-5MS column in the scan mode from 40 to 500 amu. Gammahydroxybutyrate-d6 (GHB-d6) was used as the internal standard. The limits of detection and quantitation in 2 µL of serum ranged, respectively, from 0.7 mg/L for EG to 8.5 mg/L for TEG and from 1.3 mg/L for EG to 18.2 mg/L for 1,2-PD. A linear response was observed over the concentration range from 1 to 800 mg/L for EG and 18 from 800 for TEG and 1,2-PD for serum and blood. Coefficients of variation for both intra-assay precision and interassay reproductibility ranged respectively between 1.9% for TEG to 4.9% for 1,2-PD (11.8% for HXG) and 3.5% for DEG to 9% for 2,3-BD (20.4 for HXG) at the 400 mg/L serum level. The method was applied to plasma and whole blood.</div>
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Determination of Glycols in Biological Specimens by Gas Chromatography-Mass Spectrometry

Determination of Glycols in Biological Specimens by Gas Chromatography-Mass Spectrometry

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Abstract

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